Cloned (Comment) | Organism |
---|---|
gene gldA, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3) | Serratia marcescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits (3S/3R)-acetoin reduction | Serratia marcescens | |
Fe2+ | strongly inhibits (3S/3R)-acetoin reduction by over 85% | Serratia marcescens | |
Fe3+ | strongly inhibits (3S/3R)-acetoin reduction by over 85% | Serratia marcescens | |
Zn2+ | inhibits (3S/3R)-acetoin reduction | Serratia marcescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | GDH activity is significantly enhanced to 784% for meso-2,3-butanediol oxidation in the presence of Fe2+ | Serratia marcescens | |
Mg2+ | enhances (3S/3R)-acetoin reduction | Serratia marcescens | |
Mn2+ | GDH activity is significantly enhanced for (3S/3R)-acetoin reduction in the presence of Mn2+ | Serratia marcescens | |
Na+ | enhances (3S/3R)-acetoin reduction | Serratia marcescens | |
NH4+ | enhances (3S/3R)-acetoin reduction | Serratia marcescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, recombinant enzyme, SDS-PAGE | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
- |
- |
Serratia marcescens H30 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.48 | - |
crude enzyme, with substrate (3S/3R)-acetoin and NADH at room temperature, pH 8.0, 22°C | Serratia marcescens |
20.75 | - |
purified enzyme, with substrate (3S/3R)-acetoin and NADH at room temperature, pH 8.0, 22°C | Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R,3S)-acetoin + NADH | the enzyme shows lower Km value and higher catalytic efficiency for (3S/3R)-acetoin in comparison to those for (2R,3R)-2,3-butanediol and meso-2,3-butanediol, the reduction reaction is preferred, low activity with (2R,3R)-2,3-butanediol | Serratia marcescens | (2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+ | - |
r | |
(3R,3S)-acetoin + NADH | the enzyme shows lower Km value and higher catalytic efficiency for (3S/3R)-acetoin in comparison to those for (2R,3R)-2,3-butanediol and meso-2,3-butanediol, the reduction reaction is preferred, low activity with (2R,3R)-2,3-butanediol | Serratia marcescens H30 | (2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+ | - |
r | |
1,4-butanediol + NAD+ | low activity | Serratia marcescens | ? | - |
r | |
1,4-butanediol + NAD+ | low activity | Serratia marcescens H30 | ? | - |
r | |
diacetyl + NADH + H+ | diacetyl can be converted into 2,3-butanediol via acetoin by the enzyme | Serratia marcescens | acetoin + NAD+ | - |
? | |
diacetyl + NADH + H+ | diacetyl can be converted into 2,3-butanediol via acetoin by the enzyme | Serratia marcescens H30 | acetoin + NAD+ | - |
? | |
isopropanol + NAD+ | low activity | Serratia marcescens | ? | - |
r | |
isopropanol + NAD+ | low activity | Serratia marcescens H30 | ? | - |
r | |
additional information | the purified enzyme glycerol dehydrogenase, GDH EC 1.1.1.6, also catalyzes the interconversion of (3S)-acetoin/meso-2,3-butanediol and (3R)-acetoin/(2R,3R)-2,3-butanediol. (2S,3S)-2,3-Butanediol is not a substrate for the GDH at all. Also no activity with 1-propanol, 1-butanol, isopentanol, ethylene glycol, ethanol, and (3S/3R)-acetoin in the oxidation reaction, and poor activity with formaldehyde in the reduction reaction | Serratia marcescens | ? | - |
? | |
additional information | the purified enzyme glycerol dehydrogenase, GDH EC 1.1.1.6, also catalyzes the interconversion of (3S)-acetoin/meso-2,3-butanediol and (3R)-acetoin/(2R,3R)-2,3-butanediol. (2S,3S)-2,3-Butanediol is not a substrate for the GDH at all. Also no activity with 1-propanol, 1-butanol, isopentanol, ethylene glycol, ethanol, and (3S/3R)-acetoin in the oxidation reaction, and poor activity with formaldehyde in the reduction reaction | Serratia marcescens H30 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, recombinant enzyme, SDS-PAGE | Serratia marcescens |
Synonyms | Comment | Organism |
---|---|---|
2,3-butanediol dehydrogenase | - |
Serratia marcescens |
acetoin/diacetyl reductase | - |
Serratia marcescens |
BDH | - |
Serratia marcescens |
More | cf. EC 1.1.1.6, glycerol dehydrogenase | Serratia marcescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Serratia marcescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | temperature profile, overview | Serratia marcescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
over 60% of maximal activity is retained | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
diacetyl reduction | Serratia marcescens |
8 | - |
reduction of (3S/3R)-acetoin and oxidations of meso-2,3-butanediol and glycerol | Serratia marcescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | temperature profile, overview | Serratia marcescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Serratia marcescens | |
NADH | - |
Serratia marcescens |
General Information | Comment | Organism |
---|---|---|
evolution | the Serratia marcescens enzyme belongs to the type III Fe-ADH superfamily, three consecutive glycine residues belong to a 14-amino acid residue motif (GDK motif) as the coenzyme NAD(H) binding site, and three conserved histidine residues belong to a 16-residue segment that is homologous to the 15-residue stretch as the binding site of metal | Serratia marcescens |